Recombinant Canine CD47 Protein, with His Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP8114-100ug

  • Size

    100ug

  • Price

    $525

1
  • SKU

    YCP8114-1mg

  • Size

    1mg

  • Price

    $3,465

1
This recombinant protein contains AA Gln 19 - Asn 140 of accession number F1P6D7-1. Its calculated molecular weight is 15.8 kDa. In SDS-PAGE, the protein migrates at 27-32 kDa under reducing (R) condition due to glycosylation.
gene name CD47
alias CD47, MER6, IAP, OA3
host species Canine
production host HEK293
tags His Tag
purity >90% as determined by reduced SDS-PAGE.
endotoxin The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein in production buffer (PBS, pH7.4) was 0.22 um filtered before lyophilization. Usually mannitol or trehalose was added as protectant from lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
concentration Lyophilized Powder.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of CD47 protein

ELISA: Plate-coupled Human SIRP alpha at 5 ug/mL (100 uL/well) binds to Canine CD47 with a linear range of 5-78 ng/mL.


PRODUCT

  • SKU

    YCP8114-100ug

  • Size

    100ug

  • Price

    $525

1
  • SKU

    YCP8114-1mg

  • Size

    1mg

  • Price

    $3,465

1