Human CD47 (Gln19-Pro139) protein, with C-10xHis Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP8116-50ug

  • Size

    50ug

  • Price

    $645

1
  • SKU

    YCP8116-1mg

  • Size

    1mg

  • Price

    $4,950

1
This recombinant protein contains Gln19-Pro139 of UniProt Q08722. It has a His tag at the C-terminus. The protein's calculated molecular weight is 15.6 kDa, migrating at 35-45 kDa in reducing SDS-PAGE due to glycosylation.
gene name CD47
gene id UniProt Q08722
host species Human
production host HEK293
tags C-10xHis
purity > 95% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human CD47 protein

ELISA: Immobilized Human SIRP alpha-Fc at 5 ug/mL (100 uL/well) binds to Human CD47 Protein-His with a linear range of 2-13 ng/mL.

ELISA: Immobilized Anti-CD47 MAb4 (clone 5F9) at 2 ug/mL (100 uL/well) binds to Human CD47 Protein-His with a linear range of 0.4-3 ng/mL .


PRODUCT

  • SKU

    YCP8116-50ug

  • Size

    50ug

  • Price

    $645

1
  • SKU

    YCP8116-1mg

  • Size

    1mg

  • Price

    $4,950

1