Rat CD47 (Gln19-Lys140) protein, with C-Fc Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP8131-100ug

  • Size

    100ug

  • Price

    $435

1
  • SKU

    YCP8131-1mg

  • Size

    1mg

  • Price

    $2,970

1
This recombinant protein contains Gln19-Lys140 of UniProt P97829. It has a human IgG1 Fc tag at the C-terminus. The protein's calculated molecular weight is 40.4 kDa, migrating at 55-67 kDa under reducing (R) condition, and 100-130 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
gene name CD47
gene id UniProt P97829
host species Rat
production host HEK293
tags C-Fc
purity > 95% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in Tris with Glycine, Arginine and NaCl, pH7.5, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Rat CD47 protein

ELISA: Immobilized Mouse SIRP alpha-mFc at 10 ug/mL (100 uL/well) binds to Rat CD47-Fc with a linear range of 2-20 ng/mL.


PRODUCT

  • SKU

    YCP8131-100ug

  • Size

    100ug

  • Price

    $435

1
  • SKU

    YCP8131-1mg

  • Size

    1mg

  • Price

    $2,970

1