Cynomolgus C5 / Complement C5 (Gln19-Cys1676) protein, with C-10xHis Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP8195-100ug

  • Size

    100ug

  • Price

    $525

1
  • SKU

    YCP8195-1mg

  • Size

    1mg

  • Price

    $3,810

1
This recombinant protein contains Gln19-Cys1676 of NCBI Accession# XP_005580972.1. It has a His tag at the C-terminus.The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved alpha and beta chain. Each alpha and beta chain has a calculated MW of 73.9 kDa (beta chain) and 114.9 kDa (alpha chain), migrating at 65 kDa and 113 kDa in reducing SDS-PAGE due to glycosylation.
gene name C5
host species Cynomolgus
production host HEK293
tags C-10xHis
purity > 95% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Cynomolgus Complement C5 protein

ELISA: Immobilized Cynomolgus Complement C5-His at 2 ug/mL (100 uL/well) binds to Anti-C5a (Human IgG1) with a linear range of 0.4-6 ng/mL.

ELISA: Immobilized Cynomolgus Complement C5-His at 2 ug/mL (100 uL/well) binds to Eculizumab Biosimilar with a linear range of 0.5-31 ng/mL .


PRODUCT

  • SKU

    YCP8195-100ug

  • Size

    100ug

  • Price

    $525

1
  • SKU

    YCP8195-1mg

  • Size

    1mg

  • Price

    $3,810

1