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YCP8621-50ug
50ug
$675
YCP8621-500ug
500ug
$4,200
SARS-CoV-2 spike (S) protein can be cleaved to two subunits: S1 subunit contains a receptor-binding domain (RBD) that binds to ACE2 on cell surface; S2 subunit mediates fusion between viral and host cell membranes and induce entry of the virus. The S RBD protein plays key parts in the induction of neutralizing-antibody and T-cell responses. This protein is the full-length S protein of SARS-CoV-2, containing Val16-Pro1213 from Accession# QHD43416.1, with two induced point mutations: R683A and R685A.The protein has a calculated MW of 134.6 kDa, but appears at 170-220 kDa under reducing conditions in SDS-PAGE, due to glycosylation.
| gene name | surface glycoprotein |
| alias | S protein, Spike glycoprotein, surface glycoprotein |
| host species | SARS-CoV-2 |
| production host | HEK293 |
| tags | His |
| purity | >90% as determined by SDS-PAGE. |
| endotoxin | The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug). |
| formulation | Lyophilized from 0.22 um filtered solution in PBS, pH7.4. |
| reconstitution | Reconstitute lyophilized powder with water to a concentration of 200 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage. |
| concentration | Lyophilized Powder. |
| shipping | Shipped at ambient conditions. |
| storage | Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months. |
SDS-PAGE analysis
YCP8621-50ug
50ug
$675
YCP8621-500ug
500ug
$4,200