Recombinant SARS-CoV-2 (COVID-19) S1 protein, biotinylated, expressed from HEK293

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PRODUCT

  • SKU

    YCP8627-25ug

  • Size

    25ug

  • Price

    $570

1
  • SKU

    YCP8627-200ug

  • Size

    200ug

  • Price

    $2,355

1
SARS-CoV-2 spike (S) protein can be cleaved to two subunits: S1 subunit contains a receptor-binding domain (RBD) that binds to ACE2 on cell surface; S2 subunit mediates fusion between viral and host cell membranes and induce entry of the virus. The S RBD protein plays key parts in the induction of neutralizing-antibody and T-cell responses. This protein is the S1 subunit of SARS-CoV-2 S protein, containing Val16-Arg685 of Accession# QHD43416.1.The protein has a calculated MW of 79 kDa, but appears at 100-150 kDa under reducing conditions in SDS-PAGE, due to glycosylation. This is a biotinylated protein. The protein contains an AviTag, which has been enzymatically labled with biotin.
gene name surface glycoprotein
alias S1 protein, Spike glycoprotein Subunit 1, S glycoprotein Subunit 1, Spike protein S1
host species SARS-CoV-2
production host HEK293
tags His, Avitag
purity >90% as determined by SDS-PAGE.
endotoxin The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
concentration Lyophilized Powder.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis


PRODUCT

  • SKU

    YCP8627-25ug

  • Size

    25ug

  • Price

    $570

1
  • SKU

    YCP8627-200ug

  • Size

    200ug

  • Price

    $2,355

1