Protein L binds to immunoglobulins through L chain interaction, hence the name. The resin is manufactured by coupling recombinant Protein L to high flow agarose matrix with high efficiency single point attachment chemistry. Supplied as prepacked column.
★ Binds to wider range of antibody classes than Protein A or G (because no part of the heavy chain is involved in the binding interaction): IgG, IgM, IgA, IgE and IgD, scFv, Fab and dAb.
★ Binds to human VκI, VκIII and VκIV subtypes (but not VκII) and mouse VκI.
★ Does not interfere with the antigen-binding site of the antibody, making it useful for immunoprecipitation assays.
★ Extremely useful for purification of VLκ-containing monoclonal antibodies from culture supernatant because it does not bind bovine immunoglobulin present in culture medium.
|applications||Purification of antibodies.|
|shipping||Shipped at ambient conditions.|
|storage||Store at 4-8°C, do not freeze.|