Recombinant Human LIF, Tag-free, expressed from E.coli

PRODUCT

Recombinant Human LIF was expressed from E.coli. Its molecular weight is 19.7KD. Protein sequence: Ser23-Phe202 of Uniprot accession number P15018.

gene name	LIF
alias	LIF, Leukemia inhibitory factor, CDF, DIA, HILDA, MLPLI
host species	Human
production host	E.coli
tags	None
purity	Greater than 95% as determined by reducing SDS-PAGE.
endotoxin	Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.

formulation	Protein in [20mM PB, 150mM NaCl, 0.02% Tween 20, pH 7.4] was 0.22 um filtered before lyophilized. Usually protectant (mannitol or trehalose) was added before lyophilization.
reconstitution	Reconstitute lyophilized powder with water to no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% HSA (SDP1139) be added as a carrier protein for long term storage.
shipping	Ship at ambient conditions
storage	Lyophilized form is stable at room temperature for 3 weeks. Keep at -20°C for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.