Recombinant Human SUMO1, with N-6xHis Tag, expressed from E.coli

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PRODUCT

Recombinant Human SUMO1 was expressed from E.coli. Its molecular weight is 13.7KD. Protein sequence: Met1-Val101 of Uniprot accession number P63165.
gene name SUMO1
alias SUMO1, Small ubiquitin-like modifier 1, DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1
host species Human
production host E.coli
tags N-6xHis
purity Greater than 95% as determined by reducing SDS-PAGE.
endotoxin Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
formulation Protein in [50mM TrisHCl, 100mM NaCl, 1mM DTT, pH 8.5 .] was 0.22 um filtered before lyophilized. Usually protectant (mannitol or trehalose) was added before lyophilization.
reconstitution Reconstitute lyophilized powder with water to no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% HSA (SDP1139) be added as a carrier protein for long term storage.
shipping Ship at ambient conditions
storage Lyophilized form is stable at room temperature for 3 weeks. Keep at -20°C for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

PRODUCT