Recombinant Human VAPB was expressed from E.coli. Its molecular weight is 16KD. Protein sequence: Ala2-Pro132 of Uniprot accession number O95292.
| gene name | VAPB |
| alias | VAPB, VAMP (vesicle-associated membrane protein)-associated protein B and C, ALS8, VAMP-B, VAP-B |
| host species | Human |
| production host | E.coli |
| tags | C-6xHis |
| purity | Greater than 95% as determined by reducing SDS-PAGE. |
| endotoxin | Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
| formulation | Protein in [PBS,pH7.4] was 0.22 um filtered before lyophilized. Usually protectant (mannitol or trehalose) was added before lyophilization. |
| reconstitution | Reconstitute lyophilized powder with water to no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% HSA (SDP1139) be added as a carrier protein for long term storage. |
| shipping | Ship at ambient conditions |
| storage | Lyophilized form is stable at room temperature for 3 weeks. Keep at -20°C for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months. |