Recombinant Human TNF / TNF-alpha [Val 77 - Leu 233], with C-6xHis Tag, expressed from E.coli

PRODUCT

Recombinant Human TNF was expressed from E.coli. Its molecular weight is 18.5KD. Protein sequence: Val77-Leu233 of Uniprot accession number P01375.

gene name	TNF
alias	TNF, Tumor necrosis factor, DIF-alpha, TNFA, TNFSF2, TNLG1F, TNF
host species	Human
production host	E.coli
tags	C-6xHis
purity	Greater than 95% as determined by reducing SDS-PAGE.
endotoxin	Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.

formulation	Protein in [20mM PB?150mM NaCl,pH7.4] was 0.22 um filtered before lyophilized. Usually protectant (mannitol or trehalose) was added before lyophilization.
reconstitution	Reconstitute lyophilized powder with water to no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% HSA (SDP1139) be added as a carrier protein for long term storage.
shipping	Ship at ambient conditions
storage	Lyophilized form is stable at room temperature for 3 weeks. Keep at -20°C for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.