Recombinant Human IL12 [IL12A & IL12B Heterodimer] was expressed from HEK293. Its molecular weight is 22.5&34.7KD. Protein sequence: Arg23-Ser219&Ile23-Ser328 of Uniprot accession number P29459&P29460.
| gene name | IL12 [IL12A & IL12B Heterodimer] |
| alias | IL12 [IL12A & IL12B Heterodimer], |
| host species | Human |
| production host | HEK293 |
| tags | None |
| purity | Greater than 95% as determined by reducing SDS-PAGE. |
| endotoxin | Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
| formulation | Protein in [PBS,pH7.4] was 0.22 um filtered before lyophilized. Usually protectant (mannitol or trehalose) was added before lyophilization. |
| reconstitution | Reconstitute lyophilized powder with water to no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% HSA (SDP1139) be added as a carrier protein for long term storage. |
| shipping | Ship at ambient conditions |
| storage | Lyophilized form is stable at room temperature for 3 weeks. Keep at -20°C for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months. |