Recombinant Human UBE2D1, with N-GST Tag, expressed from E.coli

PRODUCT

Recombinant Human UBE2D1 was expressed from E.coli. Its molecular weight is 42.9KD. Protein sequence: Met1-Met147 of Uniprot accession number P51668.

gene name	UBE2D1
alias	UBE2D1, Ubiquitin conjugating enzyme E2 D1, E2(17)KB1, SFT, UBC4/5, UBCH5, UBCH5A
host species	Human
production host	E.coli
tags	N-GST
purity	Greater than 95% as determined by reducing SDS-PAGE.
endotoxin	Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.

formulation	Protein in [50mM HEPES?150mM NaCl?2mM DTT,10% Glycerin pH7.5] was 0.22 um filtered before lyophilized. Usually protectant (mannitol or trehalose) was added before lyophilization.
reconstitution	Reconstitute lyophilized powder with water to no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% HSA (SDP1139) be added as a carrier protein for long term storage.
shipping	Ship at ambient conditions
storage	Lyophilized form is stable at room temperature for 3 weeks. Keep at -20°C for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.