Recombinant Human CD47, with C-Fc Tag, expressed from HEK293

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PRODUCT

Recombinant Human CD47 was expressed from HEK293. Its molecular weight is 40.8KD. Protein sequence: Gln19-Pro139 of Uniprot accession number Q08722.
gene name CD47
alias CD47, CD47 molecule, IAP, MER6, OA3
host species Human
production host HEK293
tags C-Fc
purity Greater than 95% as determined by reducing SDS-PAGE.
endotoxin Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
formulation Protein in [20mM PB?150mM NaCl,pH7.4] was 0.22 um filtered before lyophilized. Usually protectant (mannitol or trehalose) was added before lyophilization.
reconstitution Reconstitute lyophilized powder with water to no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% HSA (SDP1139) be added as a carrier protein for long term storage.
shipping Ship at ambient conditions
storage Lyophilized form is stable at room temperature for 3 weeks. Keep at -20°C for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

PRODUCT