Recombinant Human PHLDA2, with C-6xHis Tag, expressed from E.coli

PRODUCT

Recombinant Human PHLDA2 was expressed from E.coli. Its molecular weight is 18.1KD. Protein sequence: Met1-Pro152 of Uniprot accession number Q53GA4.

gene name	PHLDA2
alias	PHLDA2, Pleckstrin homology like domain family A member 2, BRW1C, BWR1C, HLDA2, IPL, TSSC3
host species	Human
production host	E.coli
tags	C-6xHis
purity	Greater than 95% as determined by reducing SDS-PAGE.
endotoxin	Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.

formulation	Protein in [20mM Tris, 0.1M NaCl, 1mM DTT, pH 8.0] was 0.22 um filtered before lyophilized. Usually protectant (mannitol or trehalose) was added before lyophilization.
reconstitution	Reconstitute lyophilized powder with water to no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% HSA (SDP1139) be added as a carrier protein for long term storage.
shipping	Ship at ambient conditions
storage	Lyophilized form is stable at room temperature for 3 weeks. Keep at -20°C for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.