Recombinant Mouse SEMA4A / Semaphorin 4A, with C-6xHis Tag, expressed from HEK293

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Recombinant Mouse SEMA4A / Semaphorin 4A was expressed from HEK293. Its molecular weight is 72.7KD. Protein sequence: Thr33-His682 of Uniprot accession number Q62178.
gene name SEMA4A
alias SEMA4A, Sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A, AI132332, SemB, Semab
host species Mouse
production host HEK293
tags C-6xHis
purity Greater than 95% as determined by reducing SDS-PAGE.
endotoxin Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
formulation Protein in [20mM PB,150mM NaCl,pH7.4] was 0.22 um filtered before lyophilized. Usually protectant (mannitol or trehalose) was added before lyophilization.
reconstitution Reconstitute lyophilized powder with water to no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% HSA (SDP1139) be added as a carrier protein for long term storage.
shipping Ship at ambient conditions
storage Lyophilized form is stable at room temperature for 3 weeks. Keep at -20°C for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

PRODUCT