Recombinant Mouse CD274 / PD-L1 / B7-H1, with C-6xHis Tag, expressed from HEK293

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PRODUCT

Recombinant Mouse CD274 / PD-L1 / B7-H1 was expressed from HEK293. Its molecular weight is 25.6KD. Protein sequence: Phe19-Thr238 of Uniprot accession number Q9EP73.
gene name CD274
alias CD274, CD274 antigen, A530045L16Rik, B7h1, Pdcd1l1, Pdcd1lg1, Pdl1
host species Mouse
production host HEK293
tags C-6xHis
purity Greater than 95% as determined by reducing SDS-PAGE.
endotoxin Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
formulation Protein in [20mM PB, 150mM NaCl, pH 7.4] was 0.22 um filtered before lyophilized. Usually protectant (mannitol or trehalose) was added before lyophilization.
reconstitution Reconstitute lyophilized powder with water to no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% HSA (SDP1139) be added as a carrier protein for long term storage.
shipping Ship at ambient conditions
storage Lyophilized form is stable at room temperature for 3 weeks. Keep at -20°C for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

PRODUCT