Human CB1 / CNR1 Full Length Transmembrane Protein (MNP, Membrane Nanoparticle)

Home/ Products / Full-length Membrane Proteins

PRODUCT

  • SKU

    DMF1012-50ug

  • Size

    50 ug

  • Price

    $1,800

1
  • SKU

    DMF1012-100ug

  • Size

    100 ug

  • Price

    $2,400

1
Cell membrane coated nanoparticles (MNPs) harboring target full-length multi-transmembrane protein in its native form are produced by overexpression of the protein in HEK293 cells followed by membrane extraction and extrusion. The target transmembrane protein maintains its natural conformation embedded in natural cell membrane coated on nanoparticles. This human full length CB1 Protein has a MW of 52.7 kDa.
target CB1
gene name CNR1
alias CANN6, CB-R, CNR1, CB1A, CB1K5, CB1R, CNR
gene id Uniprot ID: P21554
tags None
conjugate None
host species Human
production host HEK293
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize with occasional gentle mixing. Do not use solvents with pH lower than 6.5 in subsequent experiments.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

Elisa plates were pre-coated with 0.5 ug per well purified human CB1 full length membrane nanoparticles. Serial diluted anti-CB1 monoclonal antibodysolutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-CB1 monoclonal antibody binding with CB1 full length membrane nanoparticles is 439.6 ng/ml.

FACS analysis of CB1 MNPs A. Negative Control 1: CB1 full length membrane nanoparticles samples were stained only with Goat anti-human lgG 488 secondary antibody. B. Negative Control 2: Control membrane nanoparticles samples were stained with anti-CB1 antibodyat 2 ug/ml, followed by Goat anti-human IgG 488 secondary antibody. C. Negative Control 3: CB1 full length membrane nanoparticles samples were stained with anti-CCR8 antibody (an irrelevant antibody) at 2 ug/ml, followed by Goat anti-human IgG 488 secondary antibody. D. CB1 full length membrane nanoparticles samples were stained with anti-CB1 antibodyat 2 ug/ml, followed by Goat anti-human IgG 488 secondary antibody.


PRODUCT

  • SKU

    DMF1012-50ug

  • Size

    50 ug

  • Price

    $1,800

1
  • SKU

    DMF1012-100ug

  • Size

    100 ug

  • Price

    $2,400

1