Human TSHR Full Length Transmembrane Protein (PeptiNanodisc) with C-Flag Tag

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PRODUCT

  • SKU

    DMF2987-10ug

  • Size

    10ug

  • Price

    $1,550

1
  • SKU

    DMF2987-50ug

  • Size

    50ug

  • Price

    $7,260

1
  • SKU

    DMF2987-100ug

  • Size

    100ug

  • Price

    $13,200

1
Nanodiscs consist of full-length transmembrane proteins that are embedded in lipid bilayers and stabilized by scaffold materials, such as MSPs or polymers. However, this format may not be ideal for certain cellular experiments because the lipid content tends to fuse with cell membranes. PeptiNanodisc is a lipid-free system that uses synthetic peptides to shield the hydrophobic regions of membrane proteins during purification. The resulting proteins are stable, soluble, and compatible with a wide range of cell-based functional assays. This human full length TSHR protein has a MW of 86.8 kDa.
target TSHR
gene name TSHR
alias CHNG1, hTSHR-I, LGR3
gene id Uniprot ID: P16473
tags C-Flag Tag
conjugate None
host species Human
production host HEK293
formulation Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize with occasional gentle mixing. Do not use solvents with pH lower than 6.5 in subsequent experiments.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE anaylsysis of TSHR full length protein-PeptiNanodisc

TSHR-PeptiNanodisc (0.2 ug/per well) were added into ELISA plate wells pre-coated with an anti-Flag monoclonal antibody (0.2 ug/per well) plate. Serial diluted anti-TSHR monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-TSHR monoclonal antibody binding with TSHR-PeptiNanodisc is 53.59 ng/ml.


PRODUCT

  • SKU

    DMF2987-10ug

  • Size

    10ug

  • Price

    $1,550

1
  • SKU

    DMF2987-50ug

  • Size

    50ug

  • Price

    $7,260

1
  • SKU

    DMF2987-100ug

  • Size

    100ug

  • Price

    $13,200

1