Human AQP4-M23 Full Length Transmembrane Protein (Synthetic Nanodisc) with C-Flag Tag

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PRODUCT

  • SKU

    DMF2989-10ug

  • Size

    10ug

  • Price

    $1,350

1
  • SKU

    DMF2989-50ug

  • Size

    50ug

  • Price

    $6,600

1
  • SKU

    DMF2989-100ug

  • Size

    100ug

  • Price

    $12,000

1
Synthetic nanodiscs are produced by solubilizing HEK293 cell membrane fractions overexpessing target full-length multi-transmembrane protein with synthetic polymers such as styrene-maleic acid co-polymers (SMAs), forming discoidal nanostructures with target protein intact in lipid bilayer. The nanodiscs are further purified, offering intact membrane proteins in their natural configuration. This human full length AQP4-M23 protein has a MW of 32.3 kDa.
target AQP4-M23
gene name AQP4
alias MIWC, WCH4
gene id Uniprot ID: P55087-2
tags C-Flag Tag
conjugate None
host species Human
production host HEK293
formulation Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize with occasional gentle mixing. Do not use solvents with pH lower than 6.5 in subsequent experiments.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE anaylsysis of AQP4-M23 synthetic nanodisc

Elisa plates were coated with C-Flag tagged AQP4-M23-Nanodisc (0.2 ug/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with AQP4-M23-Nanodisc is 2.632 ng/ml.


PRODUCT

  • SKU

    DMF2989-10ug

  • Size

    10ug

  • Price

    $1,350

1
  • SKU

    DMF2989-50ug

  • Size

    50ug

  • Price

    $6,600

1
  • SKU

    DMF2989-100ug

  • Size

    100ug

  • Price

    $12,000

1