Human AQP4-M23 Full Length Transmembrane Protein (PeptiNanodisc) with C-Flag Tag

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PRODUCT

  • SKU

    DMF2990-10ug

  • Size

    10ug

  • Price

    $1,550

1
  • SKU

    DMF2990-50ug

  • Size

    50ug

  • Price

    $7,260

1
  • SKU

    DMF2990-100ug

  • Size

    100ug

  • Price

    $13,200

1
Nanodiscs consist of full-length transmembrane proteins that are embedded in lipid bilayers and stabilized by scaffold materials, such as MSPs or polymers. However, this format may not be ideal for certain cellular experiments because the lipid content tends to fuse with cell membranes. PeptiNanodisc is a lipid-free system that uses synthetic peptides to shield the hydrophobic regions of membrane proteins during purification. The resulting proteins are stable, soluble, and compatible with a wide range of cell-based functional assays. This human full length AQP4-M23 protein has a MW of 32.3 kDa.
target AQP4-M23
gene name AQP4
alias MIWC, WCH4
gene id Uniprot ID: P55087-2
tags C-Flag Tag
conjugate None
host species Human
production host HEK293
formulation Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize with occasional gentle mixing. Do not use solvents with pH lower than 6.5 in subsequent experiments.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE anaylsysis of AQP4-M23 PeptiNanodisc

Elisa plates were coated with C-Flag tagged AQP4-M23-PeptiNanodisc (0.2 ug/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with AQP4-M23-PeptiNanodisc is 1.485 ng/ml.


PRODUCT

  • SKU

    DMF2990-10ug

  • Size

    10ug

  • Price

    $1,550

1
  • SKU

    DMF2990-50ug

  • Size

    50ug

  • Price

    $7,260

1
  • SKU

    DMF2990-100ug

  • Size

    100ug

  • Price

    $13,200

1