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DMP1425-50ug
50 ug
$648
DMP1425-100ug
100 ug
$971
| gene name | CTLA4 |
| gene id | Uniprot ID: P16410 |
| alias | CTLA4, CD152 |
| host species | Human |
| production host | HEK293 |
| tags | C-mFc and 6xHis Tag |
| purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| endotoxin | No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug). |
| formulation | Lyophilized from sterile PBS, pH 7.4. Typically, 5 % - 8% trehalose is added as protectants before lyophilization. |
| reconstitution | Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage. |
| shipping | Shipped at ambient conditions. |
| storage | Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months. |
Human CTLA-4 Protein, mFc-His Tag, SDS-PAGE analysis under reducing conditions.
ELISA plate coated with 2 ug/ml (100 ul/well) Human B7-1, hFc tagged protein binds to Human CTLA4, mFc-His tagged protein in a linear range of 0.048-2.094 ng/ml.
ELISA plate coated with 2 ug/ml (100 ul/well) Human B7-2, hFc tagged protein binds to Human CTLA4, mFc-His tagged protein in a linear range of 0.048-2.694 ng/ml.
ELISA plate coated with 2 ug/ml (100 ul/well) Human CTLA-4, mFc-His tagged protein binds to anti-CTLA-4 neutralizing antibody in a linear range of 0.13-16.0 ng/ml.
HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CTLA4, mFc-His tagged protein 1ug/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.
Flow cytometry analysis of 1ug/ml Human CTLA4 Protein, mFc-His tag on Expi293 cells transfected with human B7-1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
DMP1425-50ug
50 ug
$648
DMP1425-100ug
100 ug
$971