Human CTLA4/CTLA-4 (Gln36-Ser161) protein, with C-hFc Tag, expressed from HEK293

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PRODUCT

  • SKU

    DMP1426-50ug

  • Size

    50 ug

  • Price

    $648

1
  • SKU

    DMP1426-100ug

  • Size

    100 ug

  • Price

    $971

1
This recombinant protein contains Gln36-Ser161 of Uniprot ID P16410. It has a C-hFc Tag, with a calculated molecular weight of 39.6 kDa. In SDS-PAGE, the protein migrates at 40-55 kDa due to glycosylation.
gene name CTLA4
gene id Uniprot ID: P16410
alias CTLA4, CD152
host species Human
production host HEK293
tags C-hFc Tag
purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Lyophilized from sterile PBS, pH 7.4. Typically, 5 % - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

ELISA plate coated with 2 ug/ml (100 ul/well) Human CTLA-4, hFc tagged protein binds to anti-CTLA4 neutralizing antibody in a linear range of 0.64-80.0 ng/ml.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CTLA4, hFc tagged protein 1ug/ml followed by Alexa 488-conjugated anti-human IgG secondary antibody.

Flow cytometry analysis of 1ug/ml Human CTLA4 Protein, hFc tag on Expi293 cells transfected with human B7-1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Human CTLA-4 Protein, hFc Tag, SDS-PAGE analysis under reducing conditions.


PRODUCT

  • SKU

    DMP1426-50ug

  • Size

    50 ug

  • Price

    $648

1
  • SKU

    DMP1426-100ug

  • Size

    100 ug

  • Price

    $971

1