Human PDCD1/PD-1 (Leu25-Gln167) protein, with C-hFc and 6xHis Tag, expressed from HEK293

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PRODUCT

  • SKU

    DMP1976-50ug

  • Size

    50 ug

  • Price

    $648

1
  • SKU

    DMP1976-100ug

  • Size

    100 ug

  • Price

    $971

1
This recombinant protein contains Leu25-Gln167 of Uniprot ID Q15116. It has a C-hFc and 6xHis Tag, with a calculated molecular weight of 42.9 kDa.
gene name PDCD1
gene id Uniprot ID: Q15116
alias PDCD1, PD1, CD279, SLEB2
host species Human
production host HEK293
tags C-hFc and 6xHis Tag
purity The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Lyophilized from sterile PBS, pH 7.4. Typically, 5 % - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

ELISA plate coated with 2 ug/ml (100 ul/well) Human PD1, hFc-His tagged protein binds to Human PDL1, mFc-His tagged protein in a linear range of 62.5-251.1 ng/ml.

Flow cytometry analysis of 0.2 ug/ml Human PD-1 Protein, hFc-His tag on Expi293 cells transfected with human PD-L1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

HEK293 cell line transfected with irrelevant protein (red histogram) and human PD-L2 protein (blue histogram)were surface stained with 2ug/ml Human PD-1 Protein, hFc-His tag followed by PE-conjugated Goat anti-human IgG secondary antibody.

Human PD-1 Protein, hFc-His Tag, SDS-PAGE analysis under reducing conditions.


PRODUCT

  • SKU

    DMP1976-50ug

  • Size

    50 ug

  • Price

    $648

1
  • SKU

    DMP1976-100ug

  • Size

    100 ug

  • Price

    $971

1