Human PDCD1 / PD-1 (Leu25-Gln167) protein, with C-Twin-Strep Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP5735-25ug

  • Size

    25ug

  • Price

    $390

1
  • SKU

    YCP5735-250ug

  • Size

    250ug

  • Price

    $1,320

1
  • SKU

    YCP5735-1mg

  • Size

    1mg

  • Price

    $3,795

1
This recombinant protein contains Leu25-Gln167 of UniProt Q15116. It has a twin strep tag at the C-terminus. The protein's calculated molecular weight is 19.0 kDa, migrating at 30-45 kDa in reducing SDS-PAGE due to glycosylation.
gene name PDCD1
gene id UniProt Q15116
host species Human
production host HEK293
tags C-Twin-Strep
purity > 90% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human PD-1 protein

ELISA: Immobilized Human PD-1 Protein, Twin-Strep Tag at 2 ug/mL (100 uL/well) binds to Human PD-L1-Fc with a linear range of 4-63 ng/mL.

ELISA: Immobilized Human PD-1 Protein, Twin-Strep Tag at 2 ug/mL (100 uL/well) binds to Human PD-L2-Fc with a linear range of 0.4-6 ng/mL .


PRODUCT

  • SKU

    YCP5735-25ug

  • Size

    25ug

  • Price

    $390

1
  • SKU

    YCP5735-250ug

  • Size

    250ug

  • Price

    $1,320

1
  • SKU

    YCP5735-1mg

  • Size

    1mg

  • Price

    $3,795

1