Recombinant rabbit anti-human CXADR chimeric monoclonal antibody, clone DMC687

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PRODUCT

  • SKU

    DMR1158-100ug

  • Size

    100ug

  • Price

    $669

1
  • SKU

    DMR1158-500ug

  • Size

    500ug

  • Price

    $2,010

1
This recombinant rabbit monoclonal antibody is part of a collection of rabbit mAbs against druggable targets developed by direct cloning from immunized rabbit B cells without hybridoma fusion. All antibodies have been fully sequenced and extensively validated by FACS and ELISA assays. Typically, multiple clones against each target are available for customer testing. The clones are available for licensing once the druggability of the antibodies has been validated by the customer, saving tremendous development time and effort. Antibody gene libraries from antigen-positive B cells are also available for further screening if the listed antibodies do not meet the customer's needs.
target CXADR
alias CAR, CAR4:6, HCAR
gene id Uniprot ID: P78310
host species Rabbit
production host HEK293
reactivity Human
clonality Monoclonal
clone number DMC687
iso type Rabbit/Human Fc chimeric IgG1
conjugate Unconjugated
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
applications Flow Cytometry, WB, IHC
dilution Flow Cyt 1:100; WB 1:1000; IHC 1:100
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

CXADR protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-CXADR (DMC687) on Expi293 cells transfected with human CXADR (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).

A. DMR1158 at 10 ug/ml staining CXADR in Huh7 xenografts in nude mice by IHC; B. The antibody at 10 ug/ml staining CXADR in human tonsil tissue by IHC

Anti-CXADR antibody at 1/1000 dilution. Lanes: Huh7, whole cell lysate. Secondary antibody: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution. Predicted band size: 40 kDa, observed band size: 50 kDa

Flow cytometry analysis of antigen binding of anti-human CXADR mAb. (A) The antibody does not bind to K562 cells that do not express CXADR. (B) A clear peak shift of The antibody was seen compared to the control when incubated with CXADR-expressing Huh7 cells, indicating strong binding of The antibody to CXADR. Antibodies were incubated at 5 ug/mL.

Related Products

PRODUCT

  • SKU

    DMR1158-100ug

  • Size

    100ug

  • Price

    $669

1
  • SKU

    DMR1158-500ug

  • Size

    500ug

  • Price

    $2,010

1

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