Recombinant rabbit anti-human TNFRSF18 / GITR monoclonal antibody, clone DM78

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PRODUCT

  • SKU

    DMR1387-100ug

  • Size

    100ug

  • Price

    $669

1
  • SKU

    DMR1387-500ug

  • Size

    500ug

  • Price

    $2,010

1
This recombinant rabbit monoclonal antibody is part of a collection of rabbit mAbs against druggable targets developed by direct cloning from immunized rabbit B cells without hybridoma fusion. All antibodies have been fully sequenced and extensively validated by FACS and ELISA assays. Typically, multiple clones against each target are available for customer testing. The clones are available for licensing once the druggability of the antibodies has been validated by the customer, saving tremendous development time and effort. Antibody gene libraries from antigen-positive B cells are also available for further screening if the listed antibodies do not meet the customer's needs.
target TNFRSF18 / GITR
alias AITR, GITR, TNFRSF18, CD357
gene id Uniprot ID: Q9Y5U5
host species Rabbit
production host HEK293
reactivity Human
clonality Monoclonal
clone number DM78
iso type Rabbit IgG
conjugate Unconjugated
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
applications ELISA, Flow Cytometry
dilution ELISA 1:5000-10000; Flow Cyt 1:100
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human GITR protein, hFc-His tagged protein binds to Rabbit anti-GITR monoclonal antibody (clone: DM78) in a linear range of 1-100 ng/ml.

Affinity ranking of different Rabbit anti-GITR mAb clones by titration of different concentration onto PC3 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Flow cytometry data of serially titrated Rabbit anti-GITR monoclonal antibody (clone: DM78) on PC3 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Flow cytometry analysis of antigen binding of rabbit anti-human GITR mAb. (A) The antibody does not bind to 293T cells that do not express GITR. (B) A clear peak shift of The antibody was seen compared to the control when incubated with GITR-expressing 8226 cells, indicating strong binding of The antibody to GITR. Antibodies were incubated at 2 ug/mL.

HEK293 cell line transfected with irrelevant protein (A) and human GITR (B) were surface stained with Rabbit anti-GITR monoclonal antibody 1ug/ml (clone: DM78) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Related Products

PRODUCT

  • SKU

    DMR1387-100ug

  • Size

    100ug

  • Price

    $669

1
  • SKU

    DMR1387-500ug

  • Size

    500ug

  • Price

    $2,010

1

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