Recombinant rabbit anti-human TNFRSF8 / CD30 monoclonal antibody, clone DM105

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PRODUCT

  • SKU

    DMR1397-100ug

  • Size

    100ug

  • Price

    $669

1
  • SKU

    DMR1397-500ug

  • Size

    500ug

  • Price

    $2,010

1
This recombinant rabbit monoclonal antibody is part of a collection of rabbit mAbs against druggable targets developed by direct cloning from immunized rabbit B cells without hybridoma fusion. All antibodies have been fully sequenced and extensively validated by FACS and ELISA assays. Typically, multiple clones against each target are available for customer testing. The clones are available for licensing once the druggability of the antibodies has been validated by the customer, saving tremendous development time and effort. Antibody gene libraries from antigen-positive B cells are also available for further screening if the listed antibodies do not meet the customer's needs.
target TNFRSF8 / CD30
alias TNFRSF8, CD30, D1S166E, Ki-1
gene id Uniprot ID: P28908
host species Rabbit
production host HEK293
reactivity Human
clonality Monoclonal
clone number DM105
iso type Rabbit IgG
conjugate Unconjugated
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
applications ELISA, Flow Cytometry, WB
dilution ELISA 1:5000-10000; Flow Cyt 1:100; WB 1:1000
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

Flow cytometry analysis with Anti-CD30 (DM105) on Expi293 cells transfected with human CD30 (Blue histogram)or Expi293 transfected with irrelevant protein (Red histogram).

Anti-CD30 antibody at 1/1000 dilution. Lanes: RPMI8226, whole cell lysate. Secondary antibody: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution. Predicted band size: 64 kDa, observed band size: 90, 110 kDa

ELISA plate pre-coated with2 ug/ml (100 ul/well) Human CD30 protein with His tagged protein PME100481 can bind to Rabbit anti-CD30 monoclonal antibody (clone: DM105) in a linear range of 0.12-80 ng/ml.

Flow cytometry analysis of antigen binding of rabbit anti-human CD30 mAb. (A) The antibody does not bind to 293T cells that do not express CD30. (B) A clear peak shift of The antibody was seen compared to the control when incubated with CD30-expressing 8226 cells, indicating strong binding of The antibody to CD30. Antibodies were incubated at 10 ug/mL.

Related Products

PRODUCT

  • SKU

    DMR1397-100ug

  • Size

    100ug

  • Price

    $669

1
  • SKU

    DMR1397-500ug

  • Size

    500ug

  • Price

    $2,010

1

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