Recombinant rabbit anti-human CDH17 chimeric monoclonal antibody, clone 5F5

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PRODUCT

  • SKU

    DMR1663-100ug

  • Size

    100ug

  • Price

    $669

1
  • SKU

    DMR1663-500ug

  • Size

    500ug

  • Price

    $2,010

1
This recombinant rabbit monoclonal antibody is part of a collection of rabbit mAbs against druggable targets developed by direct cloning from immunized rabbit B cells without hybridoma fusion. All antibodies have been fully sequenced and extensively validated by FACS and ELISA assays. Typically, multiple clones against each target are available for customer testing. The clones are available for licensing once the druggability of the antibodies has been validated by the customer, saving tremendous development time and effort. Antibody gene libraries from antigen-positive B cells are also available for further screening if the listed antibodies do not meet the customer's needs.
target CDH17
alias CDH16, HPT-1, HPT1
gene id Uniprot ID: Q12864
host species Rabbit
production host HEK293
reactivity Human
clonality Monoclonal
clone number 5F5
iso type Rabbit/Human Fc chimeric IgG1
conjugate Unconjugated
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
applications WB, Flow Cytometry
dilution WB 1:1000; Flow Cyt 1:100
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

Anti-CDH17 antibody at 1/1000 dilution. Lanes 1: AGS, whole cell lysateLane 2: HT55, whole cell lysate. Secondary antibody: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution. Predicted band size: 92 kDa, observed band size: 120 kDa

Flow cytometry analysis of antigen binding of anti-human CDH17 mAb. (A) The antibody does not bind to 293T cells that do not express CDH17 (B) A clear peak shift of The antibody was seen compared to the control when incubated with CDH17 -expressing HT55 cells, indicating strong binding of The antibody to CDH17. Antibodies were incubated at 2ug/mL.


PRODUCT

  • SKU

    DMR1663-100ug

  • Size

    100ug

  • Price

    $669

1
  • SKU

    DMR1663-500ug

  • Size

    500ug

  • Price

    $2,010

1