Recombinant rabbit anti-human GPRC5D monoclonal antibody, clone DM61, Biotinylated

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PRODUCT

  • SKU

    DMR1894-100ug

  • Size

    100ug

  • Price

    $986

1
  • SKU

    DMR1894-500ug

  • Size

    500ug

  • Price

    $2,956

1
This recombinant rabbit monoclonal antibody is part of a collection of rabbit mAbs against druggable targets developed by direct cloning from immunized rabbit B cells without hybridoma fusion. All antibodies have been fully sequenced and extensively validated by FACS and ELISA assays. Typically, multiple clones against each target are available for customer testing. The clones are available for licensing once the druggability of the antibodies has been validated by the customer, saving tremendous development time and effort. Antibody gene libraries from antigen-positive B cells are also available for further screening if the listed antibodies do not meet the customer's needs.
target GPRC5D
gene id Uniprot ID: Q9NZD1
host species Rabbit
production host HEK293
reactivity Human
clonality Monoclonal
clone number DM61
iso type Rabbit IgG
conjugate Biotinylated
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
applications ELISA,IHC
dilution ELISA 1:5000 IHC 1:100
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

DMR1894 at 10 ug/ml staining GPRC5D in in human tonsil tissue by IHC

ELISA plate pre-coated with2 ug/ml (100 ul/well) Human GPRC5D, mFc tagged protein can bind to Biotinylated Anti-GPRC5D antibody (DM61) in a linear range of 0.488-2000 ng/ml

Immunohistochemical analysis of paraffin-embedded RPMI8226 transplanted tumor labeling biotinylated Anti-GPRC5D antibody at 10ug/ml , followed by Streptavidin (HRP) ready to use. Membranous staining on RPMI8226 cells. Counter stained with Hematoxylin.Control: Used Anti-GPRC5D antibody instead of primary antibody, secondary antibody is Streptavidin (HRP) Ready to use.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Related Products

PRODUCT

  • SKU

    DMR1894-100ug

  • Size

    100ug

  • Price

    $986

1
  • SKU

    DMR1894-500ug

  • Size

    500ug

  • Price

    $2,956

1

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