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WZA1450-100ul
100uL
$865
| target | SV2A |
| host | Mouse |
| gene id | 9900 |
| reactivity | Human, Rat |
| clonality | Monoclonal |
| clone number | K70011_7D11 |
| iso type | IgG2a |
| conjugate | Unconjugated |
| applications | IHC, ICC, IP, ELISA |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | IHC 1:250-1:500, IP 1:100, ELISA 1:250-1:500 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
ELISA: Microtiter wells were coated with K70011_7D11 at 3 ug/mL as the capture antibody. SV2A (Cat. PP-4515) was used as the antigen. Peroxidase conjugated mouse anti-human SV2A monoclonal antibody (K70011_2A4) was used as the detection antibody. Result: K70011_7D11 and K70011_2A4 can be used as a matched antibody pair to detect and quantify the concentration of SV2A.
Immunohistochemistry: IHC-P analysis of brain tissue by anti-SV2A antibody (K70011_7D11). IHC-P was performed using sections of the formalin-fixed paraffin-embedded brain tissue.
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug K70011_7D11 with rat brain tissue lysate containing 200 ug total protein. After absorption with Protein G beads the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. Anti-SV2A (K70011_11A02) at 1 ug/mL was used as the primary antibody and peroxidase-conjugated goat anti-mouse IgG was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: Rat brain tissue lysate Lane 2: SV2A immunoprecipitated from rat brain tissue lysate by K70011_7D11 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K70011_7D11 can immunoprecipitate SV2A.
WZA1450-100ul
100uL
$865