Home/ Products / Mouse Monoclonal Abs
WZA1551-100ul
100uL
$650
| target | CREB1 |
| host | Mouse |
| gene id | 1385 |
| reactivity | Human, Mouse |
| clonality | Monoclonal |
| clone number | K40043_13E6 |
| iso type | IgG1 |
| conjugate | Unconjugated |
| applications | IHC, ICC, IP, WB |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | IHC 1:200, WB 1:1000, IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunohistochemistry: IHC-P analysis of brain tissue by CREB1 antibody (K40043_13E6). IHC-P was performed using sections of the formalin-fixed paraffin-embedded brain tissue.
Immunohistochemistry: IHC-P analysis of mouse brain tissue by CREB1 antibody (K40043_13E6). IHC-P was performed using sections of the formalin-fixed paraffin-embedded mouse brain tissue.
Immunohistochemistry: IHC-P analysis of testis tissue by CREB1 antibody (K40043_13E6). IHC-P was performed using sections of the formalin-fixed paraffin-embedded testis tissue.
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of K40043_13E6 with U-2 OS lysate containing 200 ug of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. CREB1 (K40043_13E6) at 1 ug/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: U-2 OS lysate Lane 2: CREB1 immunoprecipitated from U-2 OS lysate by K40043_13E6 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K40043_13E6 can immunoprecipitate CREB1.
Western Blot: Various protein samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K40043_13E6 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. CREB1 band was visualized using ECL Western Blotting Substrate. Lane 1: 15 ug of U-2 OS lysate Lane 2: 15 ug of NIH/3T3 lysate Lane 3: 15 ug of mouse brain tissue lysate Result: K40043_13E6 can detect CREB1 by Western blotting.
WZA1551-100ul
100uL
$650