Home/ Products / Mouse Monoclonal Abs
WZA1910-100ul
100uL
$540
| target | CXCL12 |
| host | Mouse |
| gene id | 6387 |
| reactivity | Human |
| clonality | Monoclonal |
| clone number | K92033_2F2 |
| iso type | IgG1 |
| conjugate | Unconjugated |
| applications | WB, ELISA |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | WB 1:1000, ELISA 1:250-1:500 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
ELISA: Microtiter wells were coated with K92033_2F2 at 3.0 ug/mL as the capture antibody. CXCL12 (Cat. PP-3056) was used as the antigen. Peroxidase conjugated mouse anti-human CXCL12 monoclonal antibody (K92033_9D8) was used as the detection antibody. Result: K92033_2F2 and K92033_9D8 can be used as a matched antibody pair to detect and quantify the concentration of CXCL12 .
Western Blot: 25 ng of recombinant human CXCL12 (Cat. PP-3056) was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K92033_2F2 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. CXCL12 band was visualized using ECL Western Blotting Substrate. Result: K92033_2F2 can detect human CXCL12 by Western blotting.
WZA1910-100ul
100uL
$540