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WZA1930-100ul
100uL
$795
| target | B2M |
| host | Mouse |
| gene id | 567 |
| reactivity | Human |
| clonality | Monoclonal |
| clone number | KT134 |
| iso type | IgG1 |
| conjugate | Unconjugated |
| applications | IP, WB, ELISA |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | WB 1:1000, IP 1:100, ELISA 1:250-1:500 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
ELISA: Microtiter wells were coated with KT134 at 3.0 ug/mL as the capture antibody. B2M (Cat. PP-11275) was used as the antigen. Peroxidase conjugated mouse anti-human B2M monoclonal antibody (KT135) was used as the detection antibody. Result: KT134 and KT135 can be used as a matched antibody pair to detect and quantify the concentration of B2M .
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug KT134 with HeLa lysate containing 200 ug total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. B2M (KT134) at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: HeLa lysate Lane 2: B2M immunoprecipitated from HeLa lysate by KT134 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: KT134 can immunoprecipitate B2M.
Western Blot: 15 ug of HeLa lysate was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. KT134 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. B2M band was visualized using ECL Substrate. Result: KT134 can detect B2M by Western blotting.
WZA1930-100ul
100uL
$795