Home/ Products / Mouse Monoclonal Abs
WZA2018-100ul
100uL
$650
| target | AFP |
| host | Mouse |
| gene id | 174 |
| reactivity | Human |
| clonality | Monoclonal |
| clone number | KT136 |
| iso type | IgG2a |
| conjugate | Unconjugated |
| applications | IP, WB |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | WB 1:1000, IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug KT136 with Hep G2 lysate containing 200 ug total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. AFP (K06092_10A11) at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: Hep G2 lysate Lane 2: AFP immunoprecipitated from Hep G2 lysate by KT136 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: KT136 can immunoprecipitate AFP.
Western Blot: 15 ug of Hep G2 lysate and 15 ug of human plasma were run on 6-18% SDS-PAGE under reducing conditions and blotted onto PVDF membrane. KT136 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. AFP band was visualized using ECL Substrate. Lane 1: 15 ug of Hep G2 lysate Lane 2: 15 ug of human plasma Result: KT136 can detect human AFP by Western blotting.
WZA2018-100ul
100uL
$650