Home/ Products / Mouse Monoclonal Abs
WZA2434-100ul
100uL
$650
| target | IFIH1 |
| host | Mouse |
| gene id | 64135 |
| reactivity | Human |
| clonality | Monoclonal |
| clone number | K49007_11A7 |
| iso type | IgG2a |
| conjugate | Unconjugated |
| applications | IP, WB |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | WB 1:1000, IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug K49007_11A7 with LPS treated THP-1 lysate containing 200 ug total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. IFIH1 (K49007_11A7) at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: LPS treated THP-1 lysate Lane 2: IFIH1 immunoprecipitated from LPS treated THP-1 lysate by K49007_11A7 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K49007_11A7 can immunoprecipitate IFIH1.
Western Blot: Cell lysate of THP-1 (untreated or treated with LPS) was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K49007_11A7 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. IFIH1 band was visualized using ECL Substrate. Lane 1: 15 ug of untreated THP-1 lysate Lane 2: 15 ug of LPS treated THP-1 lysate Result: K49007_11A7 can detect IFIH1 by Western blotting.
WZA2434-100ul
100uL
$650