Home/ Products / Mouse Monoclonal Abs
WZA3039-100ul
100uL
$540
| target | TRIM49 |
| host | Mouse |
| gene id | 57093 |
| reactivity | Human |
| clonality | Monoclonal |
| clone number | K29064_7C1 |
| iso type | IgG1 |
| conjugate | Unconjugated |
| applications | IHC, ICC, IP |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | IHC 1:200-1:250, IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunohistochemistry: IHC-P analysis of testis tissue by TRIM49 antibody (K29064_7C1). IHC-P was performed using sections of the formalin-fixed paraffin-embedded testis tissue.
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of K29064_7C1 with 200 ug of cell lysate extracted from HEK-293 transfected with DYKDDDDK tagged TRIM49 at N-terminus. After absorption with Protein G beads the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. Anti-DYKDDDDK tag antibody (K11016_5D8) at 1 ug/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: HEK-293 lysate (transfected with DYKDDDDK tagged TRIM49 (Cat. P67967QATN) at N-terminus) Lane 2: TRIM49 immunoprecipitated by K29064_7C1 from the same lysate as Lane 1 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K29064_7C1 can immunoprecipitate TRIM49.
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of K29064_7C1 with 200 ug of cell lysate extracted from HEK-293 transfected with EGFP tagged TRIM49 at C-terminus. After absorption with Protein G beads,the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. Anti-EGFP tag antibody (KAA134_2H3) at 1 ug/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: HEK-293 lysate (transfected with EGFP tagged TRIM49 (Cat. P67967QBTN) at C-terminus) Lane 2: TRIM49 immunoprecipitated by K29064_7C1 from the same lysate as Lane 1 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K29064_7C1 can immunoprecipitate TRIM49.
WZA3039-100ul
100uL
$540