Home/ Products / Mouse Monoclonal Abs
WZA4153-100ul
100uL
$650
| target | GAP43 |
| host | Mouse |
| gene id | 2596 |
| reactivity | Human, Rat |
| clonality | Monoclonal |
| clone number | K29024_1C6 |
| iso type | IgG2b |
| conjugate | Unconjugated |
| applications | IP, WB |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | WB 1:1000, IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug K29024_1C6 with rat brain tissue lysate containing 200 ug total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. Anti- GAP43 (K29024_4D6) at 1 ug/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: Rat brain tissue lysate Lane 2: GAP43 immunoprecipitated from rat brain tissue lysate by K29024_1C6 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K29024_1C6 can immunoprecipitate GAP43.
Western Blot: 15 ug of rat brain tissue lysate was run on 6-18% SDS-PAGE under reducing conditions and blotted onto PVDF membrane. K29024_1C6 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. GAP43 band was visualized using ECL Substrate. Result: K29024_1C6 can detect GAP43 by Western blotting.
WZA4153-100ul
100uL
$650