Home/ Products / Mouse Monoclonal Abs
WZA4838-100ul
100uL
$720
| target | CHEK1 |
| host | Mouse |
| gene id | 1111 |
| reactivity | Human |
| clonality | Monoclonal |
| clone number | K52027_20D11 |
| iso type | IgG2b |
| conjugate | Unconjugated |
| applications | IHC, ICC, IP |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | IHC 1:100, IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunohistochemistry: IHC-P analysis of testis tissue by CHEK1 antibody (K52027_20D11). IHC-P was performed using sections of the formalin-fixed paraffin-embedded testis tissue.
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of K52027_20D11 with MOLT- 4 lysate containing 200 ug of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. Anti- CHEK1 (K52027_2A4) at 1 ug/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: MOLT-4 lysate Lane 2: CHEK1 immunoprecipitated from MOLT-4 lysate by K52027_20D11 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result:K52027_20D11 can immunoprecipitate CHEK1.
WZA4838-100ul
100uL
$720