SARS-CoV-2 Nucleocapsid (Met1-Ala419) protein, expressed from HEK293, Biotinylated

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PRODUCT

  • SKU

    YCD1930-25ug

  • Size

    25ug

  • Price

    $570

1
  • SKU

    YCD1930-200ug

  • Size

    200ug

  • Price

    $2,595

1
This recombinant protein contains Met1-Ala419 of UniProt A0A6B9VLF5. It has a C-6xHis and C-Avi tag and was expressed from HEK293. The protein's calculated molecular weight is 49.0 kDa, but migrates at 55-65 kDa under reducing conditions in SDS-PAGE due to glycosylation.
host species SARS-CoV-2
production host HEK293
tags C-6xHis and C-Avi
purity >90% as determined by SDS-PAGE.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation The protein was lyophilized from a solution of PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Biotinylated SARS-CoV-2 Nucleocapsid protein

ELISA: Immobilized Biotinylated SARS-CoV-2 Nucleocapsid protein-His-Avi at 1 ug/mL (100 uL/well) on streptavidin precoated (0.5 ug/well) plate binds to Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb with a linear range of 0.1-3 ng/mL.


PRODUCT

  • SKU

    YCD1930-25ug

  • Size

    25ug

  • Price

    $570

1
  • SKU

    YCD1930-200ug

  • Size

    200ug

  • Price

    $2,595

1