Recombinant Mouse CD27 Protein, with Mouse Fc Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP8127-100ug

  • Size

    100ug

  • Price

    $525

1
  • SKU

    YCP8127-1mg

  • Size

    1mg

  • Price

    $3,465

1
This recombinant protein contains AA Thr 21 - Arg 182 of accession number NP_001028298.1. Its calculated molecular weight is 45.1 kDa. In SDS-PAGE, the protein migrates at 55-66 kDa under reducing (R) condition due to glycosylation.
gene name CD27
alias CD27, TNFRSF7, S152, T14, Tp55
host species Mouse
production host HEK293
tags Mouse Fc
purity >95% as determined by SDS-PAGE.
endotoxin The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 0.1 EU/ug).
formulation Protein in production buffer (Tris with Glycine, Arginine and NaCl, pH7.5) was 0.22 um filtered before lyophilization. Usually mannitol or trehalose was added as protectant from lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
concentration Lyophilized Powder.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of CD27 protein

ELISA: Plate-coupled Mouse CD27 at 2 ug/mL (100 uL/well) binds to Biotinylated Human CD27 Ligand with a linear range of 1-16 ng/mL.

ELISA: Plate-coupled Mouse CD27 at 2 ug/mL (100 uL/well) binds to Biotinylated Mouse CD70 with a linear range of 1-16 ng/mL.


PRODUCT

  • SKU

    YCP8127-100ug

  • Size

    100ug

  • Price

    $525

1
  • SKU

    YCP8127-1mg

  • Size

    1mg

  • Price

    $3,465

1