Human CD47 Full Length Transmembrane Protein (Synthetic Nanodisc) with Flag Tag

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PRODUCT

  • SKU

    DMF1044-10ug

  • Size

    10 ug

  • Price

    $1,350

1
  • SKU

    DMF1044-50ug

  • Size

    50 ug

  • Price

    $6,600

1
  • SKU

    DMF1044-100ug

  • Size

    100 ug

  • Price

    $12,000

1
Synthetic nanodiscs are produced by solubilizing HEK293 cell membrane fractions overexpessing target full-length multi-transmembrane protein with synthetic polymers such as styrene-maleic acid co-polymers (SMAs), forming discoidal nanostructures with target protein intact in lipid bilayer. The nanodiscs are further purified, offering intact membrane proteins in their natural configuration. This human full length CD47 protein has a MW of 35.2 kDa.
target CD47
gene name CD47
alias IAP, MER6, OA3
gene id Uniprot ID: Q08722
tags C-Flag Tag
conjugate None
host species Human
production host HEK293
formulation Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize with occasional gentle mixing. Do not use solvents with pH lower than 6.5 in subsequent experiments.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

Elisa plates were pre-coated with Flag Tag CD47-Nanodisc (0.2 ug per well ). Serial diluted anti-CD47 monoclonal antibodysolutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-CD47 monoclonal antibody binding with CD47-Nanodisc is 2.959 ng/ml.

Human CD47-Nanodisc, Flag Tag on SDS-PAGE

Loaded Human CD47 full length protein-synthetic nanodisc on Carboxylation Biosensor, can bind Anti-CD47 Magrolimab mAb with an affinity constant of 0.17 nM as determined in SPR assay.


PRODUCT

  • SKU

    DMF1044-10ug

  • Size

    10 ug

  • Price

    $1,350

1
  • SKU

    DMF1044-50ug

  • Size

    50 ug

  • Price

    $6,600

1
  • SKU

    DMF1044-100ug

  • Size

    100 ug

  • Price

    $12,000

1