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DMS1038-100ug
100ug
$350
| note | Other commonly used names in publications refering to this antibody: Hu5F9-G4 |
| target | CD47 |
| alias | CD47, MER6, IAP |
| gene id | Uniprot ID: Q08722 |
| host species | Human |
| reactivity | Human |
| iso type | Human IgG1 kappa |
| formulation | Lyophilized from sterile PBS, pH 7.4. Typically, 5% - 8% trehalose is added as a protective agent prior to lyophilization. |
| reconstitution | Reconstitute lyophilized powder with water to a concentration of 100 ug/ml |
| applications | ELISA, Flow Cyt |
| dilution | ELISA 1:5000-10000; Flow Cyt 1:100 |
| shipping | Shipped at ambient conditions. |
| storage | Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months. |
ELISA plate pre-coated by 1 ug/ml (100 ul/well) Human CD47, mFc-His tagged protein binds to Anti-CD47 (magrolimab biosimilar?IgG1) in a linear range of 3.2-16.0 ng/ml.
Jurkat cell line were surface stained with anti-CD47 (Blue histogram) and isotype control (Red histogram) 1ug/ml followed by Alexa 488-conjugated Goat anti-Human IgG secondary antibody.
Flow cytometry analysis of antigen binding of anti-human CD47 mAb. (A) The antibody does not bind to CHO-S cells that do not express CD47. (B) A clear peak shift of The antibody was seen compared to the control when incubated with CD47-expressing 8226 cells, indicating strong binding of The antibody to CD47. Antibodies were incubated at 5 ug/mL.
DMS1038-100ug
100ug
$350