Human CCR2 Full Length Transmembrane Protein (PeptiNanodisc) with C-Flag and Avi Tag, Biotinylated

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PRODUCT

  • SKU

    DMF2258-10ug

  • Size

    10ug

  • Price

    $1,650

1
  • SKU

    DMF2258-50ug

  • Size

    50ug

  • Price

    $7,986

1
  • SKU

    DMF2258-100ug

  • Size

    100ug

  • Price

    $14,520

1
Nanodiscs consist of full-length transmembrane proteins that are embedded in lipid bilayers and stabilized by scaffold materials, such as MSPs or polymers. However, this format may not be ideal for certain cellular experiments because the lipid content tends to fuse with cell membranes. PeptiNanodisc is a lipid-free system that uses synthetic peptides to shield the hydrophobic regions of membrane proteins during purification. The resulting proteins are stable, soluble, and compatible with a wide range of cell-based functional assays. This human full length CCR2 protein has a MW of 41.9 kDa.
target CCR2
gene name CCR2
alias CC-CKR-2, CCR-2, CCR2A, CCR2B, CD192, CKR2, CKR2A, CKR2B, CMKBR2, MCP-1-R
gene id Uniprot ID: P41597
tags C-Flag and Avi Tag
conjugate Biotinylated
host species Human
production host HEK293
formulation Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize with occasional gentle mixing. Do not use solvents with pH lower than 6.5 in subsequent experiments.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE anaylsysis of biotinylated CCR2 PeptiNanodisc

Biotinylated CCR2-PeptiNanodisc were added into ELISA wells (0.2 ug/per well) pre-coated with streptavidin (0.2 ug/per well) plate. Serial diluted anti-CCR2 monoclonal antibody and secondary antibody solutions were added, washed, and incubated sequencially before Elisa reading. From above data, the EC50 for anti-CCR2 monoclonal antibody binding with Biotinylated CCR2-PeptiNanodisc is 155.7 ng/ml.


PRODUCT

  • SKU

    DMF2258-10ug

  • Size

    10ug

  • Price

    $1,650

1
  • SKU

    DMF2258-50ug

  • Size

    50ug

  • Price

    $7,986

1
  • SKU

    DMF2258-100ug

  • Size

    100ug

  • Price

    $14,520

1