Recombinant rabbit anti-human CD38 monoclonal antibody, clone DM28

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PRODUCT

  • SKU

    DMR1080-100ug

  • Size

    100ug

  • Price

    $669

1
  • SKU

    DMR1080-500ug

  • Size

    500ug

  • Price

    $2,010

1
This recombinant rabbit monoclonal antibody is part of a collection of rabbit mAbs against druggable targets developed by direct cloning from immunized rabbit B cells without hybridoma fusion. All antibodies have been fully sequenced and extensively validated by FACS and ELISA assays. Typically, multiple clones against each target are available for customer testing. The clones are available for licensing once the druggability of the antibodies has been validated by the customer, saving tremendous development time and effort. Antibody gene libraries from antigen-positive B cells are also available for further screening if the listed antibodies do not meet the customer's needs.
target CD38
alias T10, cADPr hydrolase 1
gene id Uniprot ID: P28907
host species Rabbit
production host HEK293
reactivity Human
clonality Monoclonal
clone number DM28
iso type Rabbit IgG
conjugate Unconjugated
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
applications ELISA, IHC, WB, Flow Cytometry
dilution ELISA 1:5000-10000; Flow Cyt 1:100
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

Flow cytometry analysis of antigen binding of rabbit anti-human CD38 mAb . (A) the antibody does not bind to 293T cells that do not express CD38. (B) A clear peak shift of the antibody was seen compared to the control when incubated with CD38-expressing H929 cells, indicating strong binding of the antibody to CD38. Antibodies were incubated at 5 ug/mL.

Expi 293 cell line transfected with irrelevant protein (left) and human CD38 (right) were surface stained with Rabbit anti-CD38 monoclonal antibody 1ug/ml (clone: DM28) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

A. the antibody at 5ug/ml staining CD38 in RPMI-8226 MM xenografts in NSG mice by IHC ; B: the antibody 5ug/ml staining CD38 in human tonsil tissue by IHC ;

A. DMR1080 at 10 ug/ml staining CD38 in M-NSG Daudi DiSliceX SlideSet section by IHC; B. The antibody at 10 ug/ml staining CD38 in human tonsil tissue by IHC .

Western blot analysis of CD38 protein using Anti-CD38 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H L) at 1:5000 dilution.1. MM.1S cell lysate (native CD38 protein)2. H929 cell lysate (native CD38 protein)

Related Products

PRODUCT

  • SKU

    DMR1080-100ug

  • Size

    100ug

  • Price

    $669

1
  • SKU

    DMR1080-500ug

  • Size

    500ug

  • Price

    $2,010

1

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