Recombinant rabbit anti-human EGFR monoclonal antibody, clone DM128

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PRODUCT

  • SKU

    DMR1169-100ug

  • Size

    100ug

  • Price

    $669

1
  • SKU

    DMR1169-500ug

  • Size

    500ug

  • Price

    $2,010

1
This recombinant rabbit monoclonal antibody is part of a collection of rabbit mAbs against druggable targets developed by direct cloning from immunized rabbit B cells without hybridoma fusion. All antibodies have been fully sequenced and extensively validated by FACS and ELISA assays. Typically, multiple clones against each target are available for customer testing. The clones are available for licensing once the druggability of the antibodies has been validated by the customer, saving tremendous development time and effort. Antibody gene libraries from antigen-positive B cells are also available for further screening if the listed antibodies do not meet the customer's needs.
target EGFR
alias EGFR, ERBB, ERBB1, HER1, PIG61, mENA
gene id Uniprot ID: P00533
host species Rabbit
production host HEK293
reactivity Human
clonality Monoclonal
clone number DM128
iso type Rabbit IgG
conjugate Unconjugated
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
applications ELISA, Flow Cytometry
dilution ELISA 1:5000-10000; Flow Cyt 1:100
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

ELISA plate pre-coated by 1 ug/ml (100 ul/well) Human EGFR protein, His tagged protein binds to Rabbit anti-EGFR monoclonal antibody (clone: DM128) in a linear range of 0. 1-18 ng/ml.

EGFR protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-EGFR (DM128) on Expi293 cells transfected with human EGFR (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram),and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram)

Flow cytometry analysis of antigen binding of rabbit anti-human EGFR mAb. (A) The antibody does not bind to Jurkat cells that do not express EGFR. (B) A clear peak shift of The antibody was seen compared to the control when incubated with EGFR-expressing A431 cells, indicating strong binding of The antibody to EGFR. Antibodies were incubated at 5 ug/mL.

Flow cytometry analysis of antigen binding of rabbit anti-human EGFR mAb. (A) The antibody does not bind to Jurkat cells that do not express EGFR. (B) A clear peak shift of The antibody was seen compared to the control when incubated with EGFR-expressing SNU-5 cells, indicating strong binding of The antibody to EGFR. Antibodies were incubated at 5 ug/mL.

Related Products

PRODUCT

  • SKU

    DMR1169-100ug

  • Size

    100ug

  • Price

    $669

1
  • SKU

    DMR1169-500ug

  • Size

    500ug

  • Price

    $2,010

1

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