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DMS1063-100ug
100ug
$350
| note | Other commonly used names in publications refering to this antibody: Fab C225, IMC-225 |
| target | EGFR |
| alias | EGFR, ERBB, ERBB1, HER1, PIG61, mENA |
| gene id | Uniprot ID: P00533 |
| host species | Chimeric |
| reactivity | Human |
| iso type | Human IgG1 kappa |
| formulation | Lyophilized from sterile PBS, pH 7.4. Typically, 5% - 8% trehalose is added as a protective agent prior to lyophilization. |
| reconstitution | Reconstitute lyophilized powder with water to a concentration of 100 ug/ml |
| applications | ELISA, Flow Cyt |
| dilution | ELISA 1:5000-10000 |
| shipping | Shipped at ambient conditions. |
| storage | Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months. |
ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human EGFR, His tagged protein binds to Anti-EGFR Neutralizing antibody in a linear range of 0.64-80 ng/ml.
EGFR protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-EGFR (Cetuximab) on Expi293 cells transfected with human EGFR (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram)
Flow cytometry analysis of antigen binding of anti-human EGFR mAb. (A) A clear peak shift of The antibody was seen compared to the control when incubated with EGFR-expressing Hela cells, indicating strong binding of The antibody to EGFR. Antibodies were incubated at 2 ug/mL.
Flow cytometry analysis of antigen binding of anti-human EGFR mAb. (A) The antibody does not bind to Jurkat cells that do not express EGFR. (B) A clear peak shift of The antibody was seen compared to the control when incubated with EGFR-expressing A431 cells, indicating strong binding of The antibody to EGFR. Antibodies were incubated at 5 ug/mL.
DMS1063-100ug
100ug
$350