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WZA1179-100ul
100uL
$950
| target | UCHL1 |
| host | Mouse |
| gene id | 7345 |
| reactivity | Human, Rat |
| clonality | Monoclonal |
| clone number | K49007_1G1 |
| iso type | IgG2b |
| conjugate | Unconjugated |
| applications | IHC, ICC, IP, WB, ELISA |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | IHC 1:250-1:500, WB 1:1000, IP 1:100-1:200, ELISA 1:250-1:500 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
ELISA: Microtiter wells were coated with K49007_1G1 at 3 ug/mL as the capture antibody. UCHL1 (Cat. PP-145) was used as the antigen. Peroxidase conjugated mouse anti-human UCHL1 monoclonal antibody (K52010_3D12) was used as the detection antibody. Result: K49007_1G1 and K52010_3D12 can be used as a matched antibody pair to detect and quantify the concentration of UCHL1.
Immunohistochemistry: IHC-P analysis of brain tissue by anti-UCHL1 antibody (K49007_1G1). IHC-P was performed using sections of the formalin-fixed paraffin-embedded brain tissue.
Immunoprecipitation: Immunoprecipitation was performed by incubation of 5 ug K49007_1G1 with SH-SY5Y cell lysate containing 500 ug total protein. After absorption with Protein G beads the mixture was run on 6-18% SDS-PAGE and blotted onto PVDF membrane. Anti-UCHL1 (K52010_11G12) at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: SH-SY5Y cell lysate Lane 2: UCHL1 immunoprecipitated from SH-SY5Y cell lysate by K49007_1G1 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K49007_1G1 can immunoprecipitate UCHL1.
Western Blot: SH-SY5Y lysate and rat brain tissue lysate were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K49007_1G1 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. UCHL1 band was visualized using ECL Substrate. Lane 1: 15 ug of SH-SY5Y lysate Lane 2: 15 ug of rat brain tissue lysate Result: K49007_1G1 can detect UCHL1 by Western blotting.
WZA1179-100ul
100uL
$950