Mouse anti-Human UCHL1 Monoclonal Antibody (Clone K52010_13B4)

Immunohistochemistry: IHC-P analysis of brain tissue by anti-UCHL1 antibody (K52010_13B4). IHC-P was performed using sections of the formalin-fixed paraffin-embedded brain tissue.

Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug K52010_13B4 with SH-SY5Y lysate containing 200 ug total protein. After absorption with Protein G beads the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. Anti-UCHL1 (K52010_3D12) at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: SH-SY5Y lysate Lane 2: UCHL1 immunoprecipitated from SH-SY5Y lysate by K52010_13B4 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K52010_13B4 can immunoprecipitate UCHL1.

Western Blot: Various protein samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K52010_13B4 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. UCHL1 band was visualized using ECL Substrate. Lane 1: 15 ug of SH-SY5Y lysate Lane 2: 15 ug of U-251MG lysate Lane 3: 15 ug of rat brain tissue lysate Result: K52010_13B4 can detect UCHL1 by Western blotting.