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WZA1183-100ul
100uL
$795
| target | UCHL1 |
| host | Mouse |
| gene id | 7345 |
| reactivity | Human |
| clonality | Monoclonal |
| clone number | K52010_11G12 |
| iso type | IgG2a |
| conjugate | Unconjugated |
| applications | IP, WB |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | WB 1:1000, IP 1:100-1:200 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunoprecipitation: Immunoprecipitation was performed by incubation of 5 ug K52010_11G12 with SH-SY5Y cell lysate containing 500 ug total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto PVDF membrane. Anti-UCHL1 (K52010_11G12) at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: SH-SY5Y cell lysate Lane 2: UCHL1 immunoprecipitated from SH-SY5Y cell lysate by K52010_11G12 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K52010_11G12 can immunoprecipitate UCHL1.
Western Blot: SH-SY5Y lysate and rat brain tissue lysate were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K52010_11G12 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. UCHL1 band was visualized using ECL Substrate. Lane 1: 15 ug of SH-SY5Y lysate Lane 2: 15 ug of rat brain tissue lysate Result: K52010_11G12 can detect UCHL1 by Western blotting.
WZA1183-100ul
100uL
$795